Composite

Part:BBa_K2804010:Design

Designed by: Eduardo Alejandro Moncayo Palacio   Group: iGEM18_Ecuador   (2018-10-12)


Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1086
    Illegal XhoI site found at 1739
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon-optimised for expression in Escherichia coli and curated by the restriction restriction sites EcoRI, SpeI, PstI, XbaI, NotI


Source

This part come from Gluconacetobacter xylinus genome. Accession number: AB091058

References

Yasutake, Y., Kawano, S., Tajima, K., Yao, M., Satoh, Y., Munekata, M., & Tanaka, I. (2006). Structural characterization of the Acetobacter xylinum endo‐β‐1, 4‐glucanase CMCax required for cellulose biosynthesis. Proteins: Structure, Function, and Bioinformatics, 64(4), 1069-1077.

Sunagawa, N., Fujiwara, T., Yoda, T., Kawano, S., Satoh, Y., Yao, M., ... & Dairi, T. (2013). Cellulose complementing factor (Ccp) is a new member of the cellulose synthase complex (terminal complex) in Acetobacter xylinum. Journal of bioscience and bioengineering, 115(6), 607-612.